Structure of Petunia hybrida Defensin 1, a Novel Plant Defensin with Five Disulfide Bonds†

May 1, 2014 – 08:43 am

Cover ImageInstitute for Molecular Bioscience, ARC Special Research Center for Functional and Applied Genomics, The University of Queensland, Brisbane, Queensland 4072, Australia, and Department of Biochemistry, La Trobe University, Bundoora, Victoria 3086, Australia

Biochemistry, 2003, 42 (27), pp 8214–8222

DOI: 10.1021/bi034379o

Section:

Abstract

The structure of a novel plant defensin isolated from the flowers of Petunia hybrida has been determined by 1H NMR spectroscopy. P. hybrida defensin 1 (PhD1) is a basic, cysteine-rich, antifungal protein of 47 residues and is the first example of a new subclass of plant defensins with five disulfide bonds whose structure has been determined. PhD1 has the fold of the cysteine-stabilized αβ motif, consisting of an α-helix and a triple-stranded antiparallel β-sheet, except that it contains a fifth disulfide bond from the first loop to the α-helix. The additional disulfide bond is accommodated in PhD1 without any alteration of its tertiary structure with respect to other plant defensins. Comparison of its structure with those of classic, four-disulfide defensins has allowed us to identify a previously unrecognized hydrogen bond network that is integral to structure stabilization in the family.

Citing Articles

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This article has been cited by 3 ACS Journal articles (3 most recent appear below).

  • B. López-GarcíaB. San SegundoM. Coca

    2012, 263-294

  • Antimicrobial Peptides as a Promising Alternative for Plant Disease Protection

    B. López-GarcíaB. San SegundoM. Coca

    Plants produce antimicrobial peptides (AMPs) to defend themselves against pathogens. The repertoire of AMPs synthesized by plants is extremely large, with hundreds of different AMPs in some plant species. In spite of their molecular diversity most plant ...

  • Uma Shankar SagaramJagdeep KaurDilip Shah

    2012, 317-336

      Antifungal Plant Defensins: Structure-Activity Relationships, Modes of Action, and Biotech Applications

      Uma Shankar SagaramJagdeep KaurDilip Shah

      Small cysteine-rich antimicrobial proteins known as defensins are expressed in all plants. Although structurally similar, plant defensins exhibit substantial variation in their amino acid sequences. Some of these defensins display potent antifungal ...

  • Source: pubs.acs.org


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